Analysis of PTH-Amino Acids using HPLC-UV

Analysis of PTH-Amino acids using HPLC-UV

Sample:

  1. PTH-Histidine
  2. PTH-Arginine
  3. PTH-Asparagine
  4. PTH-Glutamine
  5. PTH-Aspartic acid
  6. PTH-Glycine
  7. PTH-Glutamic acid
  8. PTH-Alamine
  9. PTH-Tyrosine
  10. PTH-Threonine
  11. PTH-Methionine
  12. PTH-Valine
  13. PTH-Proline
  14. PTH-Tryptophan
  15. PTH-Lysine
  16. PTH-Phenylalanine
  17. PTH-Isoeucine
  18. PTH-Leucine
    **Each sample concentration is 10 nmol/mL

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm (4.6 x 250 mm)
Mobile Phase:A) 25 mM NH₄H₂PO₄ (pH=2.0)
B) Acetonitrile
GradientSee more details here.
Flow Rate:0.7 mL/min
Temperature:40°C
Detection:UV at 254 nm
Mixer Volume:10 µL

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