Analysis of Fatty Acids using HPLC-UV

Analysis of Fatty Acids using HPLC-UV

Sample:

  1. Lauric acid (0.04 mg/mL)
  2. Linolenic acid (0.05 mg/mL)
  3. Myristic acid (0.04 mg/mL)
  4. Linoleic acid (0.07 mg/mL)
  5. Oleic acid (0.05 mg/mL)
  6. Stearic acid (0.04 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm (4.6 x 150 mm)
Develosil HSR C18, 3 µm (4.6 x 150 mm)
Mobile Phase:Acetonitrile/25 mM NH₄H₂PO₄ (pH=2.0) = 80/20
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 400 nm
Injection Volume:10 µL

Fatty Acids Sample Preparation:

Fatty acids were derivatized with 2-NPH (YMC Co., Ltd)

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