Analysis of Isthiocyanates using HPLC-UV

Analysis of Isothiocyanates using HPLC-UV

Sample:

  1. Ethyl Isothiocyanate (0.013 mL/mL)
  2. Allyl Isothiocyanate (0.013 mL/mL)
  3. Propyl Isothiocyanate (0.013 mL/mL)
  4. 2-Phenylethyl Isothiocyanate
    (0.013 mL/mL)
  5. Phenyl Isothiocyanate (0.013 mL/mL)

HPLC Analytical Conditions:

Column:  Develosil HSR AQ C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase: Acetonitrile/0.2% Phosphoric Acid=50/50
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 240 nm

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