Analysis of Carotenes in Carrots using HPLC-UV

Analysis of Carotenes in Carrots using HPLC-UV

Sample:

  1. α-carotene
  2. ß-carotene

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm ( 4.6 x 250 mm)
Mobile Phase:Methanol/Ethanol=90/10
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 450 nm
Injection Volume:10.0 µL

Carrot Sample Preparation:

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