Analysis of Carotenes in Carrots using HPLC-UV

Analysis of Carotenes in Carrots using HPLC-UV

Sample:

  1. α-carotene
  2. ß-carotene

HPLC Analytical Conditions:

Column:  Develosil HSR C18, 5 µm ( 4.6 x 250 mm)
Mobile Phase: Methanol/Ethanol=90/10
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 450 nm
Injection Volume: 10.0 µL

Carrot Sample Preparation:

Carrot Sample Preparation for HPLC-UV

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