Analysis of Antiepileptics using HPLC-UV

Analysis of Antiepileptics using HPLC-UV

Sample:

  1. Primidone (0.05 mg/mL)
  2. Ethosuximide (0.76 mg/mL)
  3. Carbamazepine (0.04 mg/mL)
  4. Phenytoin (0.08 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase:Fig. 1: Acetonitrile/25 mM NH₄H₂PO₄ (pH=6.7)=32/68
Fig. 2: Methanol/25 mM NH₄H₂PO₄ (pH 6.7)=50/50
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 220 nm
Injection Volume:5.0 µL

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