Analysis of Retinol (Vitamin A) using HPLC-UV

Analysis of Catechins in Tea using HPLC-UV and UHPLC-UV


  1. Retinoic Acid (68 µg/mL)
  2. Retinol (55 µg/mL)

HPLC Analytical Conditions:

Column:  Develosil HSR C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase: Acetonitrile/Water=95/5
Flow Rate: 0.5 mL/min
Temperature: 40°C
Detection: UV at 325 nm
Injection Volume: 2.0 µL

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