Analysis of Oil-Soluble Vitamins
Sample:
- Vitamin A acetate (0.60 mg/mL)
- Vitamin D3 (0.63 mg/mL)
- Vitamin E (5.21 mg/mL)
- Vitamin E acetate (5.29 mg/mL)
- Vitamin K1 (1.89 mg/mL)
|
HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil HSR C18, 5 µm |
| Mobile Phase: | Acetonitrile/Methanol=60/40 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 30°C |
| Detection: | UV at 280 nm |
| Injection Volume: | 1.0 µL |
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