Analysis of Oil-Soluble Vitamins

Analysis of Oil-soluble Vitamins using HPLC-UV

Sample:

  1. Vitamin A acetate (0.60 mg/mL)
  2. Vitamin D3 (0.63 mg/mL)
  3. Vitamin E (5.21 mg/mL)
  4. Vitamin E acetate (5.29 mg/mL)
  5. Vitamin K1 (1.89 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm
Mobile Phase:Acetonitrile/Methanol=60/40
Flow Rate:1.0 mL/min
Temperature:30°C
Detection:UV at 280 nm
Injection Volume:1.0 µL

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