Analysis of Xanthine Derivatives using HPLC-UV

Analysis of Xanthine Derivatives using HPLC-UV

Sample:

  1. Xanthine (10 µg/mL)
  2. Theobromine (18 µg/mL)
  3. Theophiline (21 µg/mL)
  4. Caffeine (38 µg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase:Methanol/Water=30/70
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 254 nm
Injection Volume:20 µL

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