Analysis of Nucleobases using HPLC-UV

Analysis of Nucleobases using HPLC-UV

Sample:

  1. Cytosine (0.38 mg/mL)
  2. Uracil (0.36 mg/mL)
  3. Cytidine (0.36 mg/mL)
  4. Uridine (0.38 mg/mL)
  5. Thymine (0.39 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR C18, 5 µm ( 4.6 x 250 mm)
Mobile Phase:Methanol/25 mM Diammonium hydrogen phosphate=5/95
Flow Rate:0.5 mL/min
Temperature:40°C
Detection:UV at 254 nm
Injection Volume:2.0 µL

Download the product brochure or browse our technical library to find additional applications and publications.