Analysis of Vitamins using the Ion Pair Method

Analysis of Vitamins using the Ion-Pair Method

Sample:

  1. Ascorbic acid (0.12 mg/mL)
  2. Nicotinamide (0.12 mg/mL)
  3. Nicotinic acid (0.11 mg/mL)
  4. Thiamin Hydrochloride (0.13 mg/mL)
  5. Pyridoxine Hydrochloride (0.12 mg/mL)
  6. Calcium (+)-Pantothenate (1.14 mg/mL)
  7. Folic acid (0.12 mg/mL)
  8. Biotin (0.61 mg/mL)
  9. Cyanocobalamin (0.11 mg/mL)
HPLC Analytical Conditions:
Column: 1. Develosil XG-C18LC, 5 µm (4.6 x 150 mm)
2. Develosil XG-C18M, 5 µm (4.6 x 150 mm)
Mobile Phase:A) 0.1% TFA in Water
B) 0.1% TFA in Methanol
Gradient:B) 0%-70% (0-20 min)
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 210 nm
Injection Volume:5 µL

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