Analysis of Catecholamines using HPLC-UV

Analysis of Catecholamines using HPLC-UV

Sample:

  1. (R)-(-) Norepinephrine (0.1 mg/mL)
  2. (R)-(-) Epinephrine (0.1 mg/mL)
  3. Dopamine (0.1 mg/mL)

HPLC Analytical Conditions:

Column: 1. Develosil XG-C1, 5 µm (4.6 x 150 mm)
2. Develosil XG-C18LC, 5 µm (4.6 x 150 mm)
3. Develosil XG-C18M, 5 µm (4.6 x 150 mm)
4. Develosil XG-C30, 5 µm (4.6 x 150 mm)
Mobile Phase:0.1M KH₂PO₄ (pH=3.0
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 280 nm
Injection Volume:2.0 µL

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