Analysis of Isoflavones using HPLC-UV

Analysis of Isoflavones of using HPLC-UV

Sample:

  1. Puerarin (0.31 mg/mL)
  2. Daidzin (0.31 mg/mL)
  3. Daidzin (0.33 mg/mL)
  4. Biochanin A (0.28 mg/mL)
  5. Ipriflavone (0.28 mg/mL)

HPLC Analytical Conditions:

Column:  Develosil XG-C18, 5 µm ( 4.6 x 150 mm)
Mobile Phase: A) 0.2% Formc Acid-Water
B)  0.2% Formic Acid- Aceteonitrile
Gradient: Further details here
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 254 nm
Injection Volume: 10 µL

Download the product brochure or browse our technical library to find additional applications and publications.