Fatty Acid Analysis using HPLC-PDA

Fatty Acid Analysis using HPLC-UV

Sample:

  1. Lauric acid (2.0 mg/mL)
  2. Linolenic acid (0.1 µL/mL)
  3. Myristic acid (4.0 mg/mL)
  4. Linoleic acid (0.2 µL/mL)
  5. Oleic acid (2.0 µL/mL)
  6. Stearic acid (2.1 mg/mL)

HPLC Analytical Conditions:

Column:  Develosil XG-C18M 5 µm ( 4.6 x 150 mm)
Mobile Phase: Acetonitrile/0.2%Phosphoric acid=80/20
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 210 nm (PDA)
Injection Volume: 10 µL

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