Analysis of 6 Acidic Compounds using HPLC-UV

Analysis of 6 Acid Compounds using HPLC-UV

Sample:

  1. Oxalic Acid (0.16 mg/mL)
  2. L(+)–Ascorbic acid (0.80 mg/mL)
  3. Maleic acid (0.08 mg/mL)
  4. Citric acid (1.46 mg/mL)
  5. Fumaric acid (0.13 mg/mL)
  6. Uric acid (0.40 mg/mL)

HPLC Analytical Conditions:

Column:  Develosil XG-C30M, 5 µm ( 4.6 x 150 mm)
Mobile Phase: 50 mM Ammonium Phosphate (pH=2.0)
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: UV at 210 nm
Injection Volume 2.0 µL

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