Analysis of Water-soluble Vitamins using HPLC-UV

Analysis of Water-soluble Vitamins using HPLC-UV

Sample:

  1. L(+)-Ascorbic acid (0.04 mg/mL)
  2. Orotic acid (0.09 mg/mL)
  3. Nicotinic acid (0.18 mg/mL)
  4. Pyridoxine HCl (0.23 mg/mL)
  5. Nicotinamide (0.22 mg/mL)
  6. Folic acid (0.22 mg/mL)

HPLC Analytical Conditions:

Column:  Develosil XG-C18M, 5 µm (4.6 x 150 mm)
Mobile Phase: Acetonitrile/50 mM Ammonium Formate (2/98)
Flow Rate: 1.0 mL/min
Temperature: 30°C
Detection: UV at 260 nm
Injection Volume: 2.0 µL

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