Analysis of Vitamin D2 and Vitamin D3

Analysis of Vitamin D2 and Vitamin D3

Sample:

  1. Vitamin D2 (1.34 mg/mL)
  2. Vitamin D3 (1.33 µL/mL)
HPLC Analytical Conditions:
Column: 1. Develosil XG-C30M, 5 µm (4.6 x 150 mm)
2. Develosil C30-UG, 5 µm (4.6 x 150 mm)
Mobile Phase: Acetonitrile
Flow Rate:1.0 mL/min
Temperature:40°C
Detection:UV at 210 nm
Injection Volume:2.0 µL

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