Analysis of Water-soluble Vitamins using HPLC-UV

Analysis of Water-soluble Vitamins using HPLC-UV

Sample:

  1. Thiamine (0.5 mg/mL)
  2. Nicotineamide (1.0 mg/mL)
  3. Riboflavin (1.0 mg/mL)
  4. Cyanocobalamin (1.0 mg/mL)
  5. Nicotinic acid (1.0 mg/mL)
  6. Ascorbic acid (3.0 mg/mL)

HPLC Analytical Conditions:

Column: Develosil ANIDIUS (4.6 x 250 mm)
Mobile Phase:A) Acetonitrile+0.1% Formic Acid
B) 0.1 Formic Acid
Gradient:Further details here
Flow Rate:0.5 mL/min
Temperature:40°C
Detection:UV at 260 nm
Injection Volume:2.0 µL

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