Analysis of Catechins in Tea using UHPLC-UV

Analysis of Catechins in Tea using HPLC-UV and UHPLC-UV

Sample:

  1. (-)-Epigallocatechin
  2. Caffeine
  3. (-)-Epigallocatechin gallate
  4. (-)-Epicatechin
  5. (-)-Epicatechin gallate

HPLC Analytical Conditions:

Column:  Develosil HSR AQ C18, 2 µm ( 2.0 x 50 mm)
Mobile Phase: A) 0.1 % Phosphoric acid
B)  Methanol
Gradient: Further details here
Flow Rate: 0.474 mL/min
Temperature: 40°C (ForcedAir)
Detection: UV at 280 nm
Mixer Volume: 10 µL

Green Tea Sample Preparation:

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