Analysis of dNTP using UHPLC-UV

Analysis of dNTP using UHPLC-UV

Sample:

  1. dCTP (0.5 mM)
  2. dTTP (0.5 mM)
  3. dGTP (0.5 mM)
  4. dATP (0.5 mM)

HPLC Analytical Conditions:

Column: Develosil HSR AQ C18, 2 µm (2.0 x 50 mm)
Mobile Phase:25 mM Ammonium Phosphate (pH=7.0)
Flow Rate:0.474 mL/min
Temperature:40°C (Forced Air)
Detection:UV at 254 nm
Injection Volume:0.08 µL

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