Analysis of Peptide using UHPLC

Analysis of Peptide using UHPLC

Sample:

  1. Gly-Tyr (0.02 mg/mL)
  2. Val-Tyr-Val (0.02 mg/mL)
  3. Met-Enkephalin (0.02 mg/mL)
  4. Anggiontensin II (0.002 mg/mL)
  5. Leu-Enkephalin (0.02 mg/mL)

HPLC Analytical Conditions:

Column: Develosil HSR AQ C18, 2 µm ( 2.0 x 50 mm)
Mobile Phase:A) Water +0.1% TFA
B) Acetonitrile + 0.1% TFA
Gradient:Further details here
Flow Rate:0.474 mL/min
Temperature:40°C
Detection:UV at 230 nm
Injection Volume:0.16 µL

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