Analysis of Ascorbic Acid using HPLC-CAD

Analysis of Ascorbic Acid

Sample:

  1. Dehydroascorbic acid (0.34 mg/mL)
  2. L(+)-Ascorbic acid (0.34 mg/mL)
  3. Isoascorbic acid (0.34 mg/mL)

HPLC Analytical Conditions:

Column:  Develosil XG-C30M, 5 µm ( 4.6 x 150 mm)
Mobile Phase: 20 mM Ammonium Formate (pH=2.0)
Flow Rate: 1.0 mL/min
Temperature: 40°C
Detection: CAD
Injection Volume: 2.0 µL

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