FlexFire UHPLC and HPLC Columns - Develosil USA

UHPLC and HPLC Columns

FlexFire delivers supreme durability and maximum flexibility.

FlexFire’s value and performance lies in its high-quality silica, the lifeblood at the heart of its creation. Applying 40 years of manufacturing expertise and an upgraded production system, Develosil has upgraded to the highest grade uniform, high-density, low-metal porous silica. With improved end-capping technology Develosil withstands the harshest of conditions and delivers use after use, beyond the highest expectations.

With its availability in multiple functional groups and particle sizes, FlexFire is carefully designed for scalability and transferability to have your whole operation covered, from R&D to QC.

Find out more about FlexFire’s robust method development and transferability.


Column Types Particle Size (µm)
Develosil FlexFire 1.6
Brand A 1.7
Brand B 1.7
Brand C 1.8
Brand D 1.9
Column Functional Group C18
Size 2.0 x 50 mm
Mobile Phase Acetonitrile / 10 mM NH4OH, pH10.5=60/40
Flow Rate 0.5 mL/min
Temperature 40°C
Detection UV 254 nm
Sample Naphthalene (0.1 mg/mL)
Injection Volume 0.16 µL


HPLC-UHPLC Method Transferability

FlexFire series provides 1.6, 2.6 and 5 µm particle sizes to fit the requirements of your analytical systems, both HPLC and UHPLC. This wide range of options allows the same line of columns to be used in different departments, such as R&D and QC (quality control).

FlexFire dramatically shortens the required time for tweaking the optimal condition from one particle size to another. This is because the excellence and homogeneity of the silica gel, end-capping, and bonding of functional groups provide predictable consistency across all of the FlexFire columns.

The figure below shows HETP and column pressure at different flow rates. Smaller particle size provides higher resolution across a wider range of flow rates.  


Pressure and HETP vs Flow Rate for FlexFire C18

Column Develosil FlexFire C18 (2.0 x 50 mm)
Mobile Phase Acetonitrile/Water = 60/40
Temperature 40°C
Detection UV 254 nm
Sample Naphthalene
Injection Volume 0.16 µL

Example of method transfer between HPLC and UHPLC

FlexFire displays the same surface area ratio of 340 m2/g, the same physical properties, and the same bonding of functional group and end-capping on three different particle size silica substrates.

As a result, by choosing the correct column dimensions users can maintain equivalent resolution while shifting between particle sizes. This great feature makes it simple to transfer methods between different particle sizes. Chromatography software can be used to determine the flow rate and gradient without trial and error, as no adjustments are necessary after the calculation.
If you are using UHPLC during R&D and HPLC during QC, FlexFire will save time previously spent re-adjusting the analytical conditions to find the best resolution. If you are planning to adopt UHPLC in the future, you can start with FlexFire 2.6 µm or 5 µm columns without worries.

FlexFire C18, 5 µm (2.0 x 150 mm)

Gradient min mL/min %A %B Curve
0.00 0.189 90 10 6
8.44 0.189 75 25 6
16.88 0.189 75 25 6
17.06 0.189 90 10 6

Injection Volume: 1.2 µL

FlexFire C18, 2.6 µm (2.0 x 50 mm)

Gradient min mL/min %A %B Curve
0.00 0.364 90 10 6
1.46 0.364 75 25 6
2.93 0.364 75 25 6
2.96 0.364 90 10 6

Injection Volume: 0.4 µL

FlexFire C18, 1.6 µm (2.0 x 50 mm)

Gradient min mL/min %A %B Curve
0.00 0.592 90 10 6
0.90 0.592 75 25 6
1.80 0.592 75 25 6
1.82 0.592 90 10 6

Injection Volume: 0.4 µL
System:Waters ACQUITY UPLC H-Class Plus
Mixer: 100 µL

Mobile Phase A) 25mM HCOONH4, pH3.0
B) Acetonitrile
Temperature 40°C
Detection UV 260 nm
  1. Gly-Tyr
  2. Val-Tyr-Val
  3. Met-Enk
  4. Angiotensin II
  5. Leu-Enk

Specifications and Line Up

Function Particle Size Surface Area Porous Size Porous Volume Carbon Load pH Range Temp Tolerance
C18 1.6, 2.6,
and 5 µm
340 m2/g 11 nm 1.0 mL/g 22.0% 1-10 < 60°C
C30 11.0%
C8 12.0%
AQ C18 8.50%
C1 5.50%
C4 wide 2.6 and 5 µm 30 nm 1.4 mL/g 5.00% < 80°C
C4 mAb 100 nm 0.60%

Pressure Tolerance

Particle Size For all Pore Sizes
1.6 µm 800 bar
2.6 µm 600 bar
5 µm 300 bar